Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab
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Fecha
2014-05-12Autor(es)
Herrera, DanielRojas, Olga Lucía
Duarte Rey, Carolina
Mantilla, Ruben Dario
Ángel Uribe, Juanita
Franco Cortés, Manuel Antonio
Autor(es) Corporativo(s)
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Tipo
Artículo de revista
ISSN
1932-6203 / 1932-6203 (Electrónico)
Páginas
1-12
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Título en inglés
Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximabAbstract
The mechanisms that contribute to the maintenance of serological memory are still unclear. Rotavirus (RV) memory B cells(mBc) are enriched in IgM+and CD27-subpopulations, which are associated with autoimmune diseases pathogenesis. Inpatients with autoimmune diseases treated with Rituximab (RTX), some autoantibodies (auto-Abs) decrease after treatment,but other auto-Abs and pathogen-specific IgG Abs remain unchanged. Thus, maintenance of autoimmune and pathogen-specific serological memory may depend on the type of antigen and/or Ab isotype evaluated. Antigen-specific mBc andantigen-specific Abs of different isotypes have not been simultaneously assessed in patients after RTX treatment. To study the relationship between mBc subpopulations and serological memory we characterized total, RV- and tetanus toxoid (TT)-specific mBc by flow cytometry in patients with autoimmune diseases before and after treatment with RTX. We also measured total, RV- and TT-Abs, and some auto-Abs by kinetic nephelometry, ELISA, and EliA tests, respectively. Minor differences were observed between the relative frequencies of RV-mBc in healthy controls and patients with autoimmune disease. After RTX treatment, naı ̈ve Bc and total, RV- and TT-specific mBc [IgM+, switched (IgA+/IgG+), IgM+only, IgD+only, and CD27-(IgA+/IgG+/IgM+)] were significantly diminished. An important decrease in total plasma IgM and minor decrease in total IgG and IgA levels were also observed. IgM rheumatoid factor, IgG anti-CCP, and IgG anti-dsDNA were significantly diminished. In contrast, RV-IgA, RV-IgG and RV-IgG1, and TT-IgG titers remained stable. In conclusion, in patients with autoimmunity, serological memory against RV and TT seem to be maintained by long-lived plasma cells, unaffected by RTX, and an important proportion of total IgM and serological memory against some auto-antigens seem to be maintained by short-lived plasma cells, dependent on mBc precursors depleted by RTX.
Fuente
PLoS ONE; Vol. 9 Núm. 5 (2014)
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