Monocyte-derived dendritic cells from chagasic patients vs healthy donors secrete differential levels of IL-10 and IL-12 when stimulated with a protein fragment of Trypanosoma cruzi heat-shock protein-70when stimulated with a protein fragment of Trypanosoma cruzi heat-shock protein-70
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Data
2008-01-08Autore
Cuellar Ávila, AdrianaSantander, Sandra Paola
Thomas, María del Carmen
Guzmán, Fanny
Gómez Gutiérrez, Alberto
López, Manuel Carlos
Puerta Bula, Concepción
Autore/i aziendale
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Tipo
Artículo de revista
ISSN
0818-9641 / 1440-1711 (Electrónico)
Pagine
255-260
Tipo di oggetto
Artículo original
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Astratto
We analyzed the effect of the truncated heat-shock protein 70 from Trypanosoma cruzi on maturation of human dendritic cells (DCs) derived from monocytes of peripheral blood mononuclear cells from healthy donors and chagasic patients. The results show that the T-HSP70 is capable of maturing human DCs inducing an increase in the expression level of the CD83, CD86 and human leukocyte antigen-DR surface markers, as well as in the secretion of interleukin (IL)-12, tumor necrosis factor-alpha (TNF-alpha) and IL-6 cytokines. Results also show the existence of a differential functional activity of matured DCs from chagasic patients vs healthy donors in response to T-HSP70 protein and to HSP-70-derived A72 peptide, as only T-HSP70-matured DCs from chagasic patients have an enhanced secretion of IL-10 and a reduced secretion of IL-12. Moreover, the addition of A72 peptide to immature DCs from chagasic patients induced an increase in the percentage of cells expressing CD83 and CD86 molecules regarding to the expression level observed by cells from healthy donors. These findings suggest that T. cruzi HSP70 protein may induce a specific maturation profile on chagasic patients' DCs, which would favor the persistence of the parasite in the human host.
Keywords
Chagas diseaseTrypanosoma cruzi heat-shock protein-7O
Maturation dendritic cell
IL-12 and IL-10 cytokines
Cellular markers
Link alla risorsa
https://search-proquest-com.ezproxy.javeriana.edu.co/docview/1786643780/fulltextPDF/7E3C200F9C8B4549PQ/7?accountid=13250Editoriale
Immunology and Cell Biology; Vol. 86 Núm. 3 (2008)
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