Monocyte-derived dendritic cells from chagasic patients vs healthy donors secrete differential levels of IL-10 and IL-12 when stimulated with a protein fragment of Trypanosoma cruzi heat-shock protein-70when stimulated with a protein fragment of Trypanosoma cruzi heat-shock protein-70

Cuellar Ávila, Adriana; Santander, Sandra Paola; Thomas, María del Carmen; Guzmán, Fanny; Gómez Gutiérrez, Alberto; López, Manuel Carlos; Puerta Bula, Concepción

dc.rights.licence	Atribución-NoComercial 4.0 Internacional	*
dc.contributor.author	Cuellar Ávila, Adriana
dc.contributor.author	Santander, Sandra Paola
dc.contributor.author	Thomas, María del Carmen
dc.contributor.author	Guzmán, Fanny
dc.contributor.author	Gómez Gutiérrez, Alberto
dc.contributor.author	López, Manuel Carlos
dc.contributor.author	Puerta Bula, Concepción
dc.date.accessioned	2019-11-18T15:30:41Z
dc.date.accessioned	2020-04-15T13:36:00Z
dc.date.available	2019-11-18T15:30:41Z
dc.date.available	2020-04-15T13:36:00Z
dc.date.created	2008-01-08
dc.identifier	https://search-proquest-com.ezproxy.javeriana.edu.co/docview/1786643780/fulltextPDF/7E3C200F9C8B4549PQ/7?accountid=13250	spa
dc.identifier.issn	0818-9641 / 1440-1711 (Electrónico)	spa
dc.identifier.uri	http://hdl.handle.net/10554/45709
dc.language	spa	spa
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	*
dc.source	Immunology and Cell Biology; Vol. 86 Núm. 3 (2008)	spa
dc.title	Monocyte-derived dendritic cells from chagasic patients vs healthy donors secrete differential levels of IL-10 and IL-12 when stimulated with a protein fragment of Trypanosoma cruzi heat-shock protein-70when stimulated with a protein fragment of Trypanosoma cruzi heat-shock protein-70	spa
dc.type	info:eu-repo/semantics/article
dc.type.hasversion	http://purl.org/coar/version/c_ab4af688f83e57aa
dc.description.quartilewos	Q2	spa
dc.description.quartilescopus	Q1	spa
dc.identifier.doi	https://doi.org/10.1038/sj.icb.7100146	spa
dc.description.tipoarticulo	Artículo original	spa
dc.description.paginas	255-260	spa
dc.format.soporte	Papel / Electrónico	spa
dc.subject.keyword	Chagas disease	spa
dc.subject.keyword	Trypanosoma cruzi heat-shock protein-7O	spa
dc.subject.keyword	Maturation dendritic cell	spa
dc.subject.keyword	IL-12 and IL-10 cytokines	spa
dc.subject.keyword	Cellular markers	spa
dc.description.abstractenglish	We analyzed the effect of the truncated heat-shock protein 70 from Trypanosoma cruzi on maturation of human dendritic cells (DCs) derived from monocytes of peripheral blood mononuclear cells from healthy donors and chagasic patients. The results show that the T-HSP70 is capable of maturing human DCs inducing an increase in the expression level of the CD83, CD86 and human leukocyte antigen-DR surface markers, as well as in the secretion of interleukin (IL)-12, tumor necrosis factor-alpha (TNF-alpha) and IL-6 cytokines. Results also show the existence of a differential functional activity of matured DCs from chagasic patients vs healthy donors in response to T-HSP70 protein and to HSP-70-derived A72 peptide, as only T-HSP70-matured DCs from chagasic patients have an enhanced secretion of IL-10 and a reduced secretion of IL-12. Moreover, the addition of A72 peptide to immature DCs from chagasic patients induced an increase in the percentage of cells expressing CD83 and CD86 molecules regarding to the expression level observed by cells from healthy donors. These findings suggest that T. cruzi HSP70 protein may induce a specific maturation profile on chagasic patients' DCs, which would favor the persistence of the parasite in the human host.	spa
dc.type.local	Artículo de revista	spa
dc.contributor.corporatename	Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana