Circulating human rotavirus specific CD4 T cells identified with a class II tetramer express the intestinal homing receptors α4β7 and CCR9
AuthorsPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Parra Ávila, Miguel Hernando
Calvo Calle, J. Mauricio
Stern, Lawrence J.
Parra López, Carlos A.
Franco Cortés, Manuel Antonio
Ángel Uribe, Juanita
0042-6822 / 1096-0341 (Electrónico)
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Using a consensus epitope prediction approach, three rotavirus (RV) peptides that induce cytokine secretion by CD4 T cells from healthy volunteers were identified. The peptides were shown to bind HLA-DRB1 ⁎ 0101 and then used to generate MHC II tetramers. RV specific T cell lines specific for one of the three peptides studied were restricted by MHC class II molecules and contained T cells that bound the tetramer and secreted cytokines upon activation with the peptide. The majority of RV and Flu tetramer + CD4 T cells in healthy volunteers expressed markers of antigen experienced T cells, but only RV specific CD4 T cells expressed intestinal homing receptors. CD4 T cells from children that received a RV vaccine, but not placebo recipients, were stained with the RV-VP6 tetramer and also expressed intestinal homing receptors. Circulating RV-specific CD4 T cells represent a unique subset that expresses intestinal homing receptors.
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Link to the resourcehttps://www-clinicalkey-es.ezproxy.javeriana.edu.co/#!/content/journal/1-s2.0-S0042682214000178
SourceVirology; Vol. 452 (2014)
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