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dc.creatorPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
dc.creatorOrdoñez Vásquez, Adriana
dc.creatorJaramillo Gómez, Lorenza María
dc.creatorDuran Correa, Camilo
dc.creatorEscamilla García, Erandi
dc.creatorGarza Ramos, Myriam Angélica de la
dc.creatorSuárez Obando, Fernando
dc.identifier.issn2090-2999 / 2090-3006 (Electrónico)spa
dc.rightsAttribution-NonCommercial 4.0 International*
dc.sourceBone Marrow Research; (2017)spa
dc.titleA reliable and reproducible model for assessing the effect of different concentrations of of 𝛼-solanine on ratbone marrow mesenchymal stem cellsspa
dc.title.englishA reliable and reproducible model for assessing the effect of different concentrations of 𝛼-solanine on ratbone marrow mesenchymal stem cellsspa
dc.description.tipoarticuloArtículo originalspa
dc.format.soportePapel / Electrónicospa
dc.subject.subjectenglishReproducible modelspa
dc.subject.subjectenglishRat bonespa
dc.subject.subjectenglishStem cellsspa
dc.description.abstractenglishAlpha-solanine (𝛼-solanine) is a glycoalkaloid present in potato (Solatium tuberosum). It has been of particular interest because of its toxicity and potential teratogenic effects that include abnormalities of the central nervous system, such as exencephaly, encephalocele, and anophthalmia. Various types of cell culture have been used as experimental models to determine the effect of 𝛼-solanine on cell physiology. The morphological changes in the mesenchymal stem cell upon exposure to 𝛼-solanine have not been established. This study aimed to describe a reliable and reproducible model for assessing the structural changes induced by exposure of mouse bone marrow mesenchymal stem cells (MSCs) to different concentrations of 𝛼-solanine for 24 h. The results demonstrate that nonlethal concentrations of a-solanine (2-6 pM) changed the morphology of the cells, including an increase in the number of nucleoli, suggesting elevated protein synthesis, and the formation of spicules. In addition, treatment with 𝛼-solanine reduced the number of adherent cells and the formation of colonies in culture. Immunophenotypic characterization and staining of MSCs are proposed as a reproducible method that allows description of cells exposed to the glycoalkaloid, 𝛼

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Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial 4.0 International