A reliable and reproducible model for assessing the effect of different concentrations of of 𝛼�-solanine on ratbone marrow mesenchymal stem cells

Ordoñez Vásquez, Adriana; Jaramillo Gómez, Lorenza María; Duran Correa, Camilo; Escamilla García, Erandi; Garza Ramos, Myriam Angélica de la; Suárez Obando, Fernando

dc.rights.licence	Atribución-NoComercial 4.0 Internacional	*
dc.contributor.author	Ordoñez Vásquez, Adriana
dc.contributor.author	Jaramillo Gómez, Lorenza María
dc.contributor.author	Duran Correa, Camilo
dc.contributor.author	Escamilla García, Erandi
dc.contributor.author	Garza Ramos, Myriam Angélica de la
dc.contributor.author	Suárez Obando, Fernando
dc.date.accessioned	2020-02-19T12:59:06Z
dc.date.accessioned	2020-04-15T13:31:18Z
dc.date.available	2020-02-19T12:59:06Z
dc.date.available	2020-04-15T13:31:18Z
dc.date.created	2017-10-22
dc.identifier	https://www.hindawi.com/journals/bmr/2017/2170306/	spa
dc.identifier.issn	2090-2999 / 2090-3006 (Electrónico)	spa
dc.identifier.uri	http://hdl.handle.net/10554/47255
dc.format	PDF	spa
dc.format.mimetype	application/pdf	spa
dc.language	spa	spa
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	*
dc.source	Bone Marrow Research; (2017)	spa
dc.title	A reliable and reproducible model for assessing the effect of different concentrations of of 𝛼-solanine on ratbone marrow mesenchymal stem cells	spa
dc.type	info:eu-repo/semantics/article
dc.type.hasversion	http://purl.org/coar/version/c_ab4af688f83e57aa
dc.description.quartilescopus	Q3	spa
dc.title.english	A reliable and reproducible model for assessing the effect of different concentrations of 𝛼-solanine on ratbone marrow mesenchymal stem cells	spa
dc.identifier.doi	https://doi.org/10.1155/2017/2170306	spa
dc.description.tipoarticulo	Artículo original	spa
dc.description.paginas	1-7	spa
dc.format.soporte	Papel / Electrónico	spa
dc.subject.keyword	Reproducible model	spa
dc.subject.keyword	Rat bone	spa
dc.subject.keyword	Stem cells	spa
dc.description.abstractenglish	Alpha-solanine (𝛼-solanine) is a glycoalkaloid present in potato (Solatium tuberosum). It has been of particular interest because of its toxicity and potential teratogenic effects that include abnormalities of the central nervous system, such as exencephaly, encephalocele, and anophthalmia. Various types of cell culture have been used as experimental models to determine the effect of 𝛼-solanine on cell physiology. The morphological changes in the mesenchymal stem cell upon exposure to 𝛼-solanine have not been established. This study aimed to describe a reliable and reproducible model for assessing the structural changes induced by exposure of mouse bone marrow mesenchymal stem cells (MSCs) to different concentrations of 𝛼-solanine for 24 h. The results demonstrate that nonlethal concentrations of a-solanine (2-6 pM) changed the morphology of the cells, including an increase in the number of nucleoli, suggesting elevated protein synthesis, and the formation of spicules. In addition, treatment with 𝛼-solanine reduced the number of adherent cells and the formation of colonies in culture. Immunophenotypic characterization and staining of MSCs are proposed as a reproducible method that allows description of cells exposed to the glycoalkaloid, 𝛼-solanine.	spa
dc.type.local	Artículo de revista	spa
dc.contributor.corporatename	Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana