Increased tissue factor and thrombomodulin expression and histopathological changes in placentas of pregnancies with preeclampsia
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Fecha
2015-10-13Autor(es)
Ayala Ramírez, Paola AndreaRodríguez Sarmiento, Jorge Luis
Olaya Contreras, Mercedes
García Robles, Reggie
Poveda Gutiérrez, Alisson Geraldine
Pineda Buitrago, Tatiana
Autor(es) Corporativo(s)
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Patología
Tipo
Artículo de revista
ISSN
1934-5798 / 1934-5798 (Electrónico)
Páginas
31-39
Tipo de artículo
Artículo original
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Título en inglés
Increased tissue factor and thrombomodulin expression and histopathological changes in placentas of pregnancies with preeclampsia.Abstract
INTRODUCTION: Preeclampsia has a global frequency of 2–8% and a frequency of 10% in developing countries. In Colombia, preeclampsia causes 42% of maternal mortality. Alterations in placental homeostasis have been proposed to be involved in its pathophysiology. The aim of this study was to compare mRNA and protein levels of tissue factor (F3) and thrombomodulin (THBD) and the histopathological findings of placentas.
MATERIALS AND METHODS: We studied 16 placentas from patients with preeclampsia and 19 term placentas with uncomplicated pregnancy. An expert pathologist, who was masked to the group assignment, conducted an evaluation to determine specific histological changes. Assessments of mRNA and protein levels of F3 and THBD were performed using real-time PCR and ELISA, respectively.
RESULTS: Cases and controls differed in the frequency of decidual arteriopathy (p = 0.027), acute infarction (p = 0.001) and hyperplasia of the syncytiotrophoblast (p = 0.0017). Cases had increased levels of F3 mRNA (p = 0.0124) and protein (p < 0.0001) and THBD mRNA (p < 0.0001) and protein (p < 0.0001).
CONCLUSION:In placenta of patients with preeclampsia, we detected abnormal expression of F3 and THBD with increased protein and mRNA levels. The role of these molecules in the pathogenesis of this disease and in alterations of hemostatic and histopathological aspects of placentas need further studying.
Fuente
Journal of Neonatal - Perinatal Medicine; Vol. 9 Núm. 1 (2015)
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