Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction
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Fecha
2012-09-30Autor(es)
Ayala Ramírez, Paola AndreaGarcía Robles, Reggie
Rojas Barrera, Juan Diego
Bermúdez de Rincón, Martha Cecilia
Bernal Villegas, Jaime Eduardo
Autor(es) Corporativo(s)
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Tipo
Artículo de revista
ISSN
0120-8322 / 1657-9534 (Electrónico)
Páginas
184-188
Tipo de artículo
Artículo original
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Título en inglés
Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restrictionAbstract
Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies.
Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase.
Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statistically significant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNA expression of hPL gene (p = 0.5785) between cases and controls.
Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.
Keywords
RCIUMessenger RNA
Plasma
Pregnancy complications
Human placental lactogen
Chain Reaction Reverse Transcriptase Polymerase
Comunidad
Mujeres en embarazoFuente
Colombia Médica; Vol. 43 Núm. 3 (2012)
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