Background: Patients with COVID-19 have a high incidence of acute kidney injury (AKI), which is associated with mortality. The objective of the study was to determine the factors associated with AKI in patients with COVID-19. Methodology: A retrospective cohort was established in two university hospitals in Bogotá, Colombia. Adults hospi‑ talized for more than 48 h from March 6, 2020, to March 31, 2021, with confrmed COVID-19 were included. The main outcome was to determine the factors associated with AKI in patients with COVID-19 and the secondary outcome was estimate the incidence of AKI during the 28 days following hospital admission. Results: A total of 1584 patients were included: 60.4% were men, 738 (46.5%) developed AKI, 23.6% were classifed as KDIGO 3, and 11.1% had renal replacement therapy. The risk factors for developing AKI during hospitalization were male sex (OR 2.28, 95% CI 1.73–2.99), age (OR 1.02, 95% CI 1.01–1.03), history of chronic kidney disease (CKD) (OR 3.61, 95% CI 2.03–6.42), High Blood Pressure (HBP) (OR 6.51, 95% CI 2.10–20.2), higher qSOFA score to the admission (OR 1.4, 95% CI 1.14–1.71), the use of vancomycin (OR 1.57, 95% CI 1.05–2.37), piperacillin/tazobactam (OR 1.67, 95% CI 1.2–2.31), and vasopressor support (CI 2.39, 95% CI 1.53–3.74). The gross hospital mortality for AKI was 45.5% versus 11.7% without AKI. Conclusions: This cohort showed that male sex, age, history of HBP and CKD, presentation with elevated qSOFA, in hospital use of nephrotoxic drugs and the requirement for vasopressor support were the main risk factors for devel‑ oping AKI in patients hospitalized for COVID-19.