Interaction of rotavirus with human peripheral blood mononuclear cells : plasmacytoid dendritic cells play a role in stimulating memory rotavirus specific T cells in vitro
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Date
2007-05-11Les auteurs
Mesa Villanueva, Martha CeciliaRodríguez Camacho, Luz Stella
Franco Cortés, Manuel Antonio
Ángel Uribe, Juanita
Auteur(s) d'entreprise
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Type
Artículo de revista
ISSN
0042-6822 / 1096-0341 (Electrónico)
Des pages
174-184
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Abstrait
We studied the interaction of RV with human peripheral blood mononuclear cells (PBMC) from adult volunteers. After exposure of PBMC to rhesus RV (RRV), T and B lymphocytes, NK cells, monocytes, and myeloid and plasmacytoid dendritic cells expressed RV non-structural proteins, at variable levels. Expression of these RV proteins was abolished if infection was done in the presence of anti-VP7 neutralizing antibodies or 10% autologous scrum. Supernatants of RRV exposed PBMC contained TNF-a, IL-6, IFN-a, IFN-y, IL-2 and IL-10. Plasmacytoid DC were found to be the main source of IFN-a production, and in their absence the production of IFN-y and the frequency of RV specific T cells that secrete IFN-y diminished. Finally, we could not detect RV-antigen associated with the PBMC or expression of RV non-structural proteins in PBMC of acutely RV-infected children. Thus, although PBMC are susceptible to the initial steps of RV infection, most PBMC of children with RV-gastrocntcritis arc not infected.
Keywords
RotavirusPlasmacytoid dendritic cells
PBMC
Interferon gamma
Interferon alpha
Children
Memory T lymphocytes
Lien vers la ressource
https://www-sciencedirect-com.ezproxy.javeriana.edu.co/science/article/pii/S0042682207002589Origine
Virology; Vol. 366 Núm. 1 (2007)
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