Interaction of rotavirus with human peripheral blood mononuclear cells : plasmacytoid dendritic cells play a role in stimulating memory rotavirus specific T cells in vitro
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Data
2007-05-11Autore
Mesa Villanueva, Martha CeciliaRodríguez Camacho, Luz Stella
Franco Cortés, Manuel Antonio
Ángel Uribe, Juanita
Autore/i aziendale
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
Tipo
Artículo de revista
ISSN
0042-6822 / 1096-0341 (Electrónico)
Pagine
174-184
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Astratto
We studied the interaction of RV with human peripheral blood mononuclear cells (PBMC) from adult volunteers. After exposure of PBMC to rhesus RV (RRV), T and B lymphocytes, NK cells, monocytes, and myeloid and plasmacytoid dendritic cells expressed RV non-structural proteins, at variable levels. Expression of these RV proteins was abolished if infection was done in the presence of anti-VP7 neutralizing antibodies or 10% autologous scrum. Supernatants of RRV exposed PBMC contained TNF-a, IL-6, IFN-a, IFN-y, IL-2 and IL-10. Plasmacytoid DC were found to be the main source of IFN-a production, and in their absence the production of IFN-y and the frequency of RV specific T cells that secrete IFN-y diminished. Finally, we could not detect RV-antigen associated with the PBMC or expression of RV non-structural proteins in PBMC of acutely RV-infected children. Thus, although PBMC are susceptible to the initial steps of RV infection, most PBMC of children with RV-gastrocntcritis arc not infected.
Keywords
RotavirusPlasmacytoid dendritic cells
PBMC
Interferon gamma
Interferon alpha
Children
Memory T lymphocytes
Link alla risorsa
https://www-sciencedirect-com.ezproxy.javeriana.edu.co/science/article/pii/S0042682207002589Editoriale
Virology; Vol. 366 Núm. 1 (2007)
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