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ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO

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dc.contributor.authorVelazco Gamboa, Carolina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.authorPuentes Corredor, Manuel; Instituto de Inmunología - HSJD, Bogotá
dc.contributor.authorMoreno García, Alberto; Instituto de Inmunología - HSJD, Bogotá
dc.contributor.authorPatarroyo Murillo, Manuel Elkin; Instituto de Inmunología - HSJD, Bogotá
dc.contributor.authorPuerta Bula, Concepción; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
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dc.date.accessioned2018-02-24T15:59:55Z
dc.date.accessioned2020-04-15T18:10:41Z
dc.date.available2018-02-24T15:59:55Z
dc.date.available2020-04-15T18:10:41Z
dc.date.created2013-05-17
dc.description.abstractThe importance of obtaining the different stages of Trypanosoma cruzi in order to recognize the antigens involved in the intracellular invasion and replication processes, makes it necessary to adapt these strains to tissue culture, especially considering the high leve! of biological, biochemical and genetic variation, which is found among the strains and clones ofthe parasite. Within the aforementioned context, in this study, the Colombian strain ofT. cruzi, M un anta, was adapted to tissue culture in Yero Cells, obtaining the principal forms ofthe parasite: trypomastigotes, amastigotes and epimastigotes. lt was observed that the liberation of the trypomastigotes occurred on the seventh day postinfection and the quantity obtained was directly proportional to the number of infecting parasites. On the other hand, the majority of the trypomastigotes observed 48 hours after washing the monolayer five days after infection, were had thick-looking shapes corresponding to the initiation ofthe trasforrnation from trypomastigotes to amastigotes.spa
dc.formatPDFspa
dc.format.mimetypeapplication/pdfspa
dc.identifierhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074
dc.identifier.issn2027-1352
dc.identifier.issn0122-7483
dc.identifier.urihttp://hdl.handle.net/10554/31443
dc.language.isoeng
dc.publisherPontificia Universidad Javerianaeng
dc.relation.citationissueUniversitas Scientiarum; Vol 4, No 1 (1997); 83-94eng
dc.relation.citationissueUniversitas Scientiarum; Vol 4, No 1 (1997); 83-94spa
dc.relation.citationissueUniversitas Scientiarum; Vol 4, No 1 (1997); 83-94por
dc.relation.urihttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074/3914
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenceAtribución-NoComercial-SinDerivadas 4.0 Internacional*
dc.subjectnullspa
dc.subjectCultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzispa
dc.subjectnullspa
dc.titleADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VEROspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.localArtículo de revistaspa

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