Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab
| dc.contributor.author | Herrera, Daniel | |
| dc.contributor.author | Rojas, Olga Lucía | |
| dc.contributor.author | Duarte Rey, Carolina | |
| dc.contributor.author | Mantilla, Ruben Dario | |
| dc.contributor.author | Ángel Uribe, Juanita | |
| dc.contributor.author | Franco Cortés, Manuel Antonio | |
| dc.contributor.corporatename | Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana | |
| dc.date.accessioned | 2020-03-06T15:36:32Z | |
| dc.date.accessioned | 2020-04-15T13:35:03Z | |
| dc.date.available | 2020-03-06T15:36:32Z | |
| dc.date.available | 2020-04-15T13:35:03Z | |
| dc.date.created | 2014-05-12 | |
| dc.description.abstractenglish | The mechanisms that contribute to the maintenance of serological memory are still unclear. Rotavirus (RV) memory B cells(mBc) are enriched in IgM+and CD27-subpopulations, which are associated with autoimmune diseases pathogenesis. Inpatients with autoimmune diseases treated with Rituximab (RTX), some autoantibodies (auto-Abs) decrease after treatment,but other auto-Abs and pathogen-specific IgG Abs remain unchanged. Thus, maintenance of autoimmune and pathogen-specific serological memory may depend on the type of antigen and/or Ab isotype evaluated. Antigen-specific mBc andantigen-specific Abs of different isotypes have not been simultaneously assessed in patients after RTX treatment. To study the relationship between mBc subpopulations and serological memory we characterized total, RV- and tetanus toxoid (TT)-specific mBc by flow cytometry in patients with autoimmune diseases before and after treatment with RTX. We also measured total, RV- and TT-Abs, and some auto-Abs by kinetic nephelometry, ELISA, and EliA tests, respectively. Minor differences were observed between the relative frequencies of RV-mBc in healthy controls and patients with autoimmune disease. After RTX treatment, naı ̈ve Bc and total, RV- and TT-specific mBc [IgM+, switched (IgA+/IgG+), IgM+only, IgD+only, and CD27-(IgA+/IgG+/IgM+)] were significantly diminished. An important decrease in total plasma IgM and minor decrease in total IgG and IgA levels were also observed. IgM rheumatoid factor, IgG anti-CCP, and IgG anti-dsDNA were significantly diminished. In contrast, RV-IgA, RV-IgG and RV-IgG1, and TT-IgG titers remained stable. In conclusion, in patients with autoimmunity, serological memory against RV and TT seem to be maintained by long-lived plasma cells, unaffected by RTX, and an important proportion of total IgM and serological memory against some auto-antigens seem to be maintained by short-lived plasma cells, dependent on mBc precursors depleted by RTX. | spa |
| dc.description.paginas | 1-12 | spa |
| dc.description.quartilescopus | Q1 | spa |
| dc.description.quartilewos | Q2 | spa |
| dc.format | spa | |
| dc.format.mimetype | application/pdf | spa |
| dc.format.soporte | Electrónico | spa |
| dc.identifier | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097087 | spa |
| dc.identifier.doi | https://doi.org/10.1371/journal.pone.0097087 | spa |
| dc.identifier.issn | 1932-6203 / 1932-6203 (Electrónico) | spa |
| dc.identifier.uri | http://hdl.handle.net/10554/47504 | |
| dc.language | spa | spa |
| dc.rights.licence | Atribución-NoComercial 4.0 Internacional | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
| dc.source | PLoS ONE; Vol. 9 Núm. 5 (2014) | spa |
| dc.title | Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab | spa |
| dc.title.english | Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab | spa |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.hasversion | http://purl.org/coar/version/c_ab4af688f83e57aa | |
| dc.type.local | Artículo de revista | spa |