Production of recombinant trichoderma reesei cellobiohydrolase ii in a new expression system based on wickerhamomyces anomalus

Díaz Rincón, Dennis J.; Duque, Ivonne; Osorio, Erika; Rodríguez López, Alexander; Espejo Mojica, Angela; Parra Giraldo, Claudia Marcela; Poutou-Piñales, Raúl A.; Alméciga Díaz, Carlos J.; Quevedo Hidalgo, Balkys

Production of recombinant trichoderma reesei cellobiohydrolase ii in a new expression system based on wickerhamomyces anomalus

dc.contributor.author	Díaz Rincón, Dennis J.
dc.contributor.author	Duque, Ivonne
dc.contributor.author	Osorio, Erika
dc.contributor.author	Rodríguez López, Alexander
dc.contributor.author	Espejo Mojica, Angela
dc.contributor.author	Parra Giraldo, Claudia Marcela
dc.contributor.author	Poutou-Piñales, Raúl A.
dc.contributor.author	Alméciga Díaz, Carlos J.
dc.contributor.author	Quevedo Hidalgo, Balkys
dc.contributor.corporatename	Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Grupo de Investigación en Enfermedades Infecciosas HUSI - PUJ
dc.date.accessioned	2020-09-01T21:27:26Z
dc.date.available	2020-09-01T21:27:26Z
dc.date.created	2017
dc.description.abstractenglish	Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14.5 U L−1. The enzyme extract showed optimum pH and temperature of 5.0–6.0 and 40°C, respectively. Enzyme kinetic parameters ( of 2.73 mM and Vmax of 23.1 µM min−1) were between the ranges of values reported for other CBHII enzymes. Finally, the results showed that an enzymatic extract of W. anomalus 54-A carrying the recombinant T. reesei CBHII allows production of reducing sugars similar to that of a crude extract from cellulolytic fungi. These results show the first report on the use of W. anomalus as a host to produce recombinant proteins. In addition, recombinant T. reesei CBHII enzyme could potentially be used in the degradation of lignocellulosic residues to produce bioethanol, based on its pH and temperature activity profile.	spa
dc.description.paginas	1-7	spa
dc.description.quartilescopus	Q3	spa
dc.format	PDF	spa
dc.format.mimetype	application/pdf	spa
dc.format.soporte	Papel / Electrónico	spa
dc.identifier	http://downloads.hindawi.com/archive/2017/6980565.pdf	spa
dc.identifier.doi	https://doi.org/10.1155/2017/6980565	spa
dc.identifier.issn	2090-0406 / 2090-0414 (Electrónico)	spa
dc.identifier.uri	http://hdl.handle.net/10554/50932
dc.language	Inglés	spa
dc.rights.licence	Atribución-NoComercial 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	*
dc.source	Enzyme research; Vol. 2017 (2017)	spa
dc.title	Production of recombinant trichoderma reesei cellobiohydrolase ii in a new expression system based on wickerhamomyces anomalus	spa
dc.type	info:eu-repo/semantics/article
dc.type.hasversion	http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.local	Artículo de revista	spa