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Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis

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dc.contributor.authorBernal Forigua, Camila
dc.contributor.authorOtálora-Otálora, Beatriz Andrea
dc.contributor.authorCanas, Alejandra
dc.contributor.authorBarreto, Alfonso
dc.contributor.authorPrieto, Karol
dc.contributor.authorMontecino, Martín
dc.contributor.authorRojas, Adriana
dc.contributor.corporatenamePontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humanaspa
dc.contributor.corporatenamePontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Neumología
dc.contributor.javerianateacherCanas, Alejandra
dc.contributor.javerianateacherRojas, Adriana
dc.coverage.cityBogotá (Colombia)spa
dc.coverage.spatialColombiaspa
dc.date.accessioned2023-08-28T14:58:45Z
dc.date.available2023-08-28T14:58:45Z
dc.date.created2022-12-03
dc.description.abstractenglishLung cancer is the leading cause of cancer death globally. Numerous factors intervene in the onset and progression of lung tumors, among which the participation of lineage-specific transcription factors stands out. Several transcription factors important in embryonic development are abnormally expressed in adult tissues and thus participate in the activation of signaling pathways related to the acquisition of the tumor phenotype. RUNX2 is the transcription factor responsible for osteogenic differentiation in mammals. Current studies have confirmed that RUNX2 is closely related to the proliferation, invasion, and bone metastasis of multiple cancer types, such as osteosarcoma, breast cancer (BC), prostate cancer, gastric cancer, colorectal cancer, and lung cancer. Thus, the present study is aimed at evaluating the role of the RUNX2 transcription factor in inhibiting the apoptosis process. Loss-of-function assays using sh-RNA from lentiviral particles and coupled with Annexin/propidium iodide (PI) assays (flow cytometry), immunofluorescence, and quantitative PCR analysis of genes related to cell apoptosis (BAD, BAX, BCL2, BCL-XL, and MCL1) were performed. Silencing assays and Annexin/PI assays demonstrated that when RUNX2 was absent, the percentage of dead cells increased, and the expression levels of the BCL2, BCL-XL, and MCL1 genes were downregulated. Furthermore, to confirm whether the regulatory role of RUNX2 in the expression of these genes is related to its binding to the promoter region, we performed chromatin immunoprecipitation (ChIP) assays. Here, we report that overexpression of the RUNX2 gene in lung cancer may be related to the inhibition of the intrinsic apoptosis pathway, specifically, through direct transcriptional regulation of the antiapoptotic gene BCL2 and indirect regulation of BCL-XL and MCL1.spa
dc.description.comunidadPacientes con Cáncer de pulmónspa
dc.description.esciNospa
dc.description.indexingRevista Internacional - Indexadaspa
dc.description.orcidhttps://orcid.org/0000-0003-1555-6661spa
dc.description.orcidhttps://orcid.org/0000-0002-5668-0266spa
dc.description.orcidhttps://orcid.org/0000-0003-3975-2835spa
dc.description.orcidhttps://orcid.org/0000-0001-8528-4433spa
dc.description.publindexCspa
dc.description.quartilescopusQ4spa
dc.formatPDFspa
dc.format.mimetypeapplication/pdfspa
dc.identifierhttps://www.hindawi.com/journals/ijcb/2022/5198203/#abstractspa
dc.identifier.doihttps://doi.org/10.1155/2022/5198203spa
dc.identifier.instnameinstname:Pontificia Universidad Javerianaspa
dc.identifier.issn1687-8876 / 1687-8884 (Electrónico)spa
dc.identifier.reponamereponame:Repositorio Institucional - Pontificia Universidad Javerianaspa
dc.identifier.repourlrepourl:https://repository.javeriana.edu.cospa
dc.identifier.urihttp://hdl.handle.net/10554/65318
dc.language.isoengspa
dc.relation.citationendpage13spa
dc.relation.citationstartpage1spa
dc.relation.ispartofjournalInternational Journal of Cell Biologyspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenceAtribución-NoComercial 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.titleRegulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosisspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.hasversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.localArtículo de revistaspa

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